CLEC10A Knockout cell line (THP-1)

ENCN
location： Home > Products > KO Cell Lines > CLEC10A Knockout cell line (THP-1)

CLEC10A Knockout cell line (THP-1) Order

CLEC10A Knockout cell line (THP-1)
Catalog#
YKO-H774
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CLEC10A Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CLEC10A Knockout cell line（THP-1）
Cell Morphology
Monocyte,suspension
Passage ratio
1：2-1：4
Species
Human
Gene Name
CLEC10A
Gene ID
10462
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Accession
CVCL_E1CT(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 4 (based on transcript CLEC10A-202)
Validation: PCR+Sanger Sequencing
CLEC10A Knockout cell line (THP-1) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:THP-1		 STR Info (Cell bank)
Cell Line:THP-1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 13131313
D7S820 10101010
D16S539 11121112
VWA 16161616
TH01 89.389.3
AMEL XYXY
TPOX 811811
CSF1PO 11131113
D12S391 1919
FGA 2425
D2S1338 1718
D21S11 3031.2
D18S51 1314
D8S1179 1014
D3S1358 1517
D6S1043 1414
PENTAE 1115
D19S433 12.213
PENTAD 1012
D1S1656 1617
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CLEC10A gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CLEC10A gene knockout CLEC10A Knockout cell line（THP-1） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
CLEC10A Knockout cell line (THP-1)
CLEC10A Knockout cell line (THP-1)
Catalog#
YKO-H774
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • Clec10a Knockout cell line (Hepa 1-6)

    Clec10a Knockout cell line (Hepa 1-6)
      inquiry   More details

    Organism: Mouse
    Gene Name: Clec10a
    Gene ID: 17312
    Size: 1*10^6
    Catalog#: YKO-M092
    YKO-M092(Hepa 1-6)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • Clec10a Knockout cell line (Hepa 1-6)

    Clec10a Knockout cell line (Hepa 1-6)

    Organism: Mouse
    Gene Name: Clec10a
    Gene ID: 17312
    Size: 1*10^6
    Catalog#: YKO-M092
      inquiry   More details
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now